Keywords:
Laser-assisted microdissection: a new tool for aquatic molecular parasitology Journal Article
M. Longshaw;S. W. Feist;G. D. Stentiford;B. P. Lyons;H. J. Small;J. Sturve;J. P. Bignell;R. Hicks
In: Diseases of Aquatic Organisms, vol. 82, no. 2, pp. 151–156, 2008, (ISBN: 0177-5103).
Pathology and parasitology Peer review Technical
@article{small_laser-assisted_2008,
title = {Laser-assisted microdissection: a new tool for aquatic molecular parasitology},
author = {H J Small and J Sturve and J P Bignell and M Longshaw and B P Lyons and R Hicks and S W Feist and G D Stentiford},
doi = {10.3354/dao01983},
year = {2008},
date = {2008-01-01},
journal = {Diseases of Aquatic Organisms},
volume = {82},
number = {2},
pages = {151–156},
abstract = {Laser-assisted microdissection (LMD) has been developed to isolate distinct cell populations from heterogeneous tissue sections, cytological preparations, or live cell samples. Downstream applications typically include gene expression studies using real-time PCR and array platforms, diagnostic PCR, and protein expression studies. LMD techniques are now commonplace in mainstream biological research and clearly have suitable applications in the field of aquatic pathology and parasitology. The present study used LMD to isolate 2 dinoflagellate parasites (Hematodinium spp.) from formalin-fixed paraffin-embedded tissue sections from 2 crustacean hosts, Cancer pagurus and Portunus trituberculatus. DNA was isolated from LMD parasite preparations, and partial regions (up to 300 bp) of the small subunit and the first internal transcribed spacer region of the rRNA gene complex from the Hematodinium spp. were PCR amplified using diagnostic primers. The amplification products were sequenced to confirm the identity of the targeted regions, The techniques, applications, and limitations of LMD to address questions in aquatic molecular pathology and parasitology are discussed},
note = {ISBN: 0177-5103},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Laser-assisted microdissection (LMD) has been developed to isolate distinct cell populations from heterogeneous tissue sections, cytological preparations, or live cell samples. Downstream applications typically include gene expression studies using real-time PCR and array platforms, diagnostic PCR, and protein expression studies. LMD techniques are now commonplace in mainstream biological research and clearly have suitable applications in the field of aquatic pathology and parasitology. The present study used LMD to isolate 2 dinoflagellate parasites (Hematodinium spp.) from formalin-fixed paraffin-embedded tissue sections from 2 crustacean hosts, Cancer pagurus and Portunus trituberculatus. DNA was isolated from LMD parasite preparations, and partial regions (up to 300 bp) of the small subunit and the first internal transcribed spacer region of the rRNA gene complex from the Hematodinium spp. were PCR amplified using diagnostic primers. The amplification products were sequenced to confirm the identity of the targeted regions, The techniques, applications, and limitations of LMD to address questions in aquatic molecular pathology and parasitology are discussed
Immunostaining of spores and plasmodia of disparate myxozoan genera with comments on the properties of the sporular mucus envelope Journal Article
M. Longshaw;D. J. Morris;K. Molnár;A. Adams
In: Parasitology, vol. 132, no. 6, pp. 781–790, 2006, ISSN: 00311820.
Pathology and parasitology Peer review Animal health Technical
@article{morris_immunostaining_2006,
title = {Immunostaining of spores and plasmodia of disparate myxozoan genera with comments on the properties of the sporular mucus envelope},
author = {D. J. Morris and K. Molnár and M. Longshaw and A. Adams},
doi = {10.1017/S0031182005009807},
issn = {00311820},
year = {2006},
date = {2006-01-01},
journal = {Parasitology},
volume = {132},
number = {6},
pages = {781–790},
abstract = {Species of the phylum Myxozoa are common parasites of fish and can cause severe losses in cultured species. Although a number of myxozoan life-cycles have now been elucidated, little is known about the biology of these organisms in the fish host. Monoclonal antibody B4 raised to the myxozoan Tetracapsuloides bryosalmonae has been previously noted to react with a number of species infecting fish kidney. We present the results of a survey of 55 myxosporean species that determined that this antibody detects an antigen on the spore surface of 33 of these species in the genera Myxobolus, Sphaerospora and Thelohanellus. However, there appears to be no clear relationship between those spores that contain the MAb B4 reactive antigen and the host or organ in which they are detected. The antigen appears to be synthesized in the plasmodial cytoplasm and is intimately associated with the surface of the spore capsules and, where present, the mucus envelope. The nature of this envelope is further discussed in relation to its formation and distinctive properties. © 2006 Cambridge University Press.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Species of the phylum Myxozoa are common parasites of fish and can cause severe losses in cultured species. Although a number of myxozoan life-cycles have now been elucidated, little is known about the biology of these organisms in the fish host. Monoclonal antibody B4 raised to the myxozoan Tetracapsuloides bryosalmonae has been previously noted to react with a number of species infecting fish kidney. We present the results of a survey of 55 myxosporean species that determined that this antibody detects an antigen on the spore surface of 33 of these species in the genera Myxobolus, Sphaerospora and Thelohanellus. However, there appears to be no clear relationship between those spores that contain the MAb B4 reactive antigen and the host or organ in which they are detected. The antigen appears to be synthesized in the plasmodial cytoplasm and is intimately associated with the surface of the spore capsules and, where present, the mucus envelope. The nature of this envelope is further discussed in relation to its formation and distinctive properties. © 2006 Cambridge University Press.